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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Cancer Res. 2016 Sep 26;76(23):6964–6974. doi: 10.1158/0008-5472.CAN-16-0258

Figure 1. Tumor-intrinsic PD-L1 controls immune-independent growth and metastatic spread.

Figure 1

Flow cytometry for PD-L1 expression of in vitro cultured cells. Interferon (IFN)-γ 0.1 ng/mL added for 48 h as indicated for (A) ID8agg or (B) B16. C. Proliferation in vitro of B16 cells determined by MTT versus control (ctrl, set at 100%). p-value, unpaired t test. D. NSG mice challenged subcutaneously with indicated B16 cells. P values for tumor size by two-way ANOVA and for survival by log-rank test. E. NSG mice challenged with indicated B16 cells and sacrificed on day 18. Genes in whole lung lysates by qPCR. p-value, unpaired t test. *, p < 0.05, **, p < 0.01. F. Proliferation in vitro of ID8agg cells as in panel C and survival in vivo (G) as in panel D after intraperitoneal ID8agg challenge.