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. 2016 Oct 14;12(6):4515–4523. doi: 10.3892/ol.2016.5261

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Copyright: © Gan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Knockdown of Aurora-A sensitizes glioblastoma cells to TMZ in vitro. U251 cells were transfected with plasmid-based shAurora-A, shCtrl or medium alone as control for 48 h. Aurora-A expression was detected by (A) western blotting or (B) reverse transcription-polymerase chain reaction analysis. GAPDH expression was monitored as the control. The ratio of Aurora-A/GAPDH was calculated using densitometry. The experiments were performed in triplicate and the mean ratio is shown. The transfected (C) U251 and (D) U87-MG cells were plated for CCK8 assay and treated with or without TMZ. DMSO was used as the vehicle control. In this analysis, the absorbance was examined at 450 nm 0, 24, 48, 72 and 96 h after cell plating (n=5). Values are presented as the mean ± standard deviation. The experiment was performed in triplicate. **P<0.01 compared with shCtrl group; ^##P<0.01 compared with TMZ group. sh, short hairpin; Ctrl, control; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; CCK8, cell counting Kit-8; TMZ, temozolomide; DMSO, dimethyl sulfoxide; OD, optical density.