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. 2016 Oct 14;12(6):4515–4523. doi: 10.3892/ol.2016.5261

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Copyright: © Gan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effects of shAurora-A on U251 invasion and tube formation. (A) Cell invasion in U251 cells was evaluated using Transwell chambers. These experiments were performed in triplicate (magnification, ×200). (B) The average number of invasive cells is shown. Values are presented as the mean ± SD. **P<0.01 compared with shCtrl group; *P<0.05 compared with shCtrl group; ^##P<0.01 compared with TMZ group. (C) Western blot analysis was performed to measure the expression of the invasion-associated proteins MMP-2 and MMP-9 in each group. (D) Conditional medium from each group was used for tube formation assays in human umbilical vein endothelial cells. These experiments were performed in triplicate (magnification, ×200). (E) The tubular structure in each group was quantified by manual counting. The mean number of branch points is shown. Values are presented as the mean ± SD. **P<0.01 compared with shCtrl group; *P<0.05 compared with shCtrl group; ^##P<0.01 compared with TMZ group. (F) VEGF expression from the conditional medium of each group was determined via enzyme-linked immunosorbent assay. These experiments were performed in triplicate. The average concentrations of VEGF are shown. Values are presented as the mean ± SD. **P<0.01 compared with shCtrl group; ^##P<0.01 compared with TMZ group. sh, short hairpin; SD, standard deviation; TMZ, temozolomide; MMP, matrix metallopeptidase; Ctrl, control; DMSO, dimethyl sulfoxide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; VEGF, vascular endothelial growth factor.