Fig. 2.

Exosomal transfer enhances recipient cell viability – (A-C) Exosomal-mediated RNA transfer into recipient cells was determined. C33A cells exposed to Hela exosomes containing normal RNA or BrUTP-labelled RNA were analysed by FACS to determine if RNA can transfer into cells from exosomes. RNA transfer was determined by FITC fluorescence in cells exposed to exosomes for (A) 24 h or (B) 48 h and (C) the percentage transfer of BrUTP-labelled exsomal RNA determined. (D) Exosomal cell viability of HEK293 cells treated in triplicate with Hela exosomes or a PBS control. Cell viability was the analysed at 48 and 72 h using the Millipore Muse cell analyser. The effects of the top candidate exosome associated RNAs expression on cell viability (E) HEK293 cells were transfected in triplicate to overexpress candidate lncRNAs (Exo1-4, and RMRP) along with a GFP control. Lipofectamine only and untransfected cells served as the controls. Cell viability was measured using the Millipore Muse cell analyser at 48 and 72 h post-transfection. For (D-E) Error bars represent standard error of the mean, *represents P < 0.05 relative to GFP Control 1.