Fig. 3.

Mechanism of exosome associated RNA regulation of cell viability and associated proteins – (A) Biotin containing transcripts Exo1-4, and RMRP were transfected into HEK293 cells and elutes from the ChIP sent for mass spec analysis at the UNSW BMFS facility in triplicate. Proteins present in at least two of the samples and not in the GFP controls were flagged as candidates of interest. (B) Relative enrichment of the candidate exosome associated RNAs at homology containing loci. Biotin tagged transcripts (Exo1, Exo2 and RMRP were transfected into HEK293 cells in triplicate and 48 h later immunoprecipitated with steptavidin beads. Enrichment of each biotin transcript is shown at homology containing loci relative to the GFP control. Error bars represent standard error of the mean,*represents p < 0.05. (C) A UCSC snapshot of the RMRP target locus found enriched by CHIP (in B above) is shown with the relative CHIP primer binding sites and associated epigenetic state of the locus.