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Production of IFN-γ in response to PPD in whole blood of infants pre- and post-BCG vaccination, unvaccinated infants, and adult controls. Triplicate whole-blood samples of infants pre (○)- and post (•)-BCG vaccination (n = 33), unvaccinated control infants (×) (n = 4), and adult controls (T1 [▴], n = 6; T2 [▾], n = 6) were diluted 1:10 with RPMI medium and stimulated with PPD (10 μg/ml). Supernatants were harvested at day 6, and IFN-γ was measured by ELISA.
