FIG. 3.

Humoral analysis of C57BL/6, IL-4 KO, IL-4/IL-10 KO, and IL-10 KO mice following infection. Results for filarial specific IgG1 (A) and IgG2a (B) are shown, representing one of two consistent experiments. For each isotype, four or five mice were bled at day 65 p.i. by retro-orbital venipuncture. Sandwich ELISA was performed to determine the filaria-specific concentration of the relevant isotype in serum. *, significant difference in IgG concentration (Mann-Whitney U test and Bonferroni correction, P = 0.0044).