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. 2004 Nov;72(11):6666–6675. doi: 10.1128/IAI.72.11.6666-6675.2004

FIG. 3.

FIG. 3.

Effects of IFN-γ treatment on C. burnetii growth in J774.16 and J774D.9 cells. At 48, 96, and 144 h postinfection, infected cells were harvested, and the numbers of C. burnetii organisms, represented as numbers of Coxiella genomes, were determined by real-time PCR. (A) J774.16 cells were infected for 24 h with virulent phase I C. burnetii and then were treated with 100 U of murine rIFN-γ/ml in the presence or absence of the iNOS inhibitor NMMLA (1 mM) or the hydrogen peroxide scavenger catalase (100 μM). (B) J774D.9 cells were infected for 24 h with virulent phase I C. burnetii and then were treated with 100 U of murine rIFN-γ/ml in the presence or absence of the iNOS inhibitor NMMLA (1 mM) or the ROI donor HX/XO (250 μM/30 μM). The results are expressed as means and standard errors for three replicates from two independent experiments. Asterisks indicate significant differences between treated and untreated samples within the same time points.