TABLE 2.
Immunolocalization of C. burnetii in mice
| Tissue or time after infection | Mouse strain | Immunolocalization of C. burnetii at indicated dosea
|
|
|---|---|---|---|
| 104 | 107 | ||
| 14 dpi | |||
| Lung | Wild type | − | − |
| iNOS KO | + | + | |
| phox KO | + | + | |
| Liver | Wild type | + | ++ |
| iNOS KO | ++ | ++ | |
| phox KO | + | +++ | |
| Spleen | Wild type | ++ | +++ |
| iNOS KO | ++++ | +++ | |
| phox KO | +++ | ++++ | |
| Heart valve | Wild type | + | + |
| iNOS KO | + | + | |
| phox KO | + | ++ | |
| 28 dpi | |||
| Lung | Wild type | − | − |
| iNOS KO | + | + | |
| phox KO | − | + | |
| Liver | Wild type | − | − |
| iNOS KO | − | − | |
| phox KO | − | + | |
| Spleen | Wild type | − | + |
| iNOS KO | + | ++ | |
| phox KO | + | ++ | |
| Heart valve | Wild type | + | + |
| iNOS KO | ++ | ++ | |
| phox KO | ++ | +++ | |
a
−, no organisms present in the tissue section; +, the presence of the organism is rare and focal; ++, few organisms are present, and they are multifocal; +++, many organisms are present, and they are diffuse; ++++, abundance of organisms are present, and they are diffuse. One section from four mice per group was evaluated. The evaluation of tissue sections was done in a blinded fashion.