FIG. 2.

PA83 domains recognized by the MAbs. (A) Schematic representation of fragments generated by cleavage of PA83 by chymotrypsin. Domain 1 (residues 1 to 258), domain 2 (residues 259 to 487), domain 3 (residues 488 to 595), and domain 4 (residues 596 to 735) are shown. (B) Chymotrypsin digestion fragments of PA83 (each, 1 μg) were subjected to electrophoresis and were transferred to a nitrocellulose membrane for Western blotting with MAbs 7.5 and 48.3 as first antibodies. (C) PA83 and PA83 proteins with mutations in domain 4 (PA608, PA705, and PA711) were absorbed onto a nitrocellulose membrane for a dot blot experiment with MAbs 7.5 and 48.3 as first antibodies.