TABLE 2.

Selected genes induced by IFN-γ but inhibited by M. tuberculosis or 19-kDa lipoprotein

Gene/GenBank accession no.	Fold inductiona
	IFN-γ	IFN-γ + 19-kDa lipoprotein	IFN-γ + H37Ra	IFN-γ + H37Rv
I-A β chain/M21932	4.7	−1.6*	−1.0*	1.7*
I-A α chain/X52643	3.4	−2.5*	−2.6*	1.4*
CD86/L25606	11.6	1.9*	3.0*	4.3*
VCAM-1/M84487	5.5	−7.8*	−1.2*	1.5*
MIG/M34815	496.5	195.5*	78.8*	186.7*
IL-12 receptor, β2/U64199	4.1	−1.3*	−1.6*	1.1*
TRAIL/U37522	13.2	4.3*	4.8*	8.1*
NKG2D/AF054819	16.7	2.3*	2.3*	7.8*
Inositol triphosphate receptor/X15373	4.2	1.3*	−1.0*	2.2*
Thromboxane A synthase/L18868	1.9	−1.3*	−1.9*	−1.3*
ALK1: TGF-b receptor type II/L48015	2.5	1.3*	1.4*	1.3*
Caspase 3/U54803	3.4	1.8*	1.2*	2.0*
Serine protease inhibitor 2/M64085	40.0	20.5*	5.6*	14.0*
Cyclin D2/M83749	23.9	1.7*	1.3*	5.2*
Ly6E.1/X04653	35.6	9.1*	25.0*	18.2*
GBP-1/M55544	489.0	48.9*	1.5*	32.2
CIITA/AF042158	3.2	1.1*	−1.0*	1.7
Chromaffin ATPase/U75321	2.4	1.7	−2.2*	−2.2*
MD-1/AB007599	2.6	1.3	−1.8*	1.5*
H-2M α-chain/U35323	6.3	1.8	1.1*	2.3*
H-2M β-chain/U35330	6.7	1.4	1.2*	2.5*
TNF receptor 1a/X57796	2.1	1.6	1.1*	1.3*
Cyclin 3/M86183	1.8	1.4	1.1*	1.2*
IL-10Rα/L12120	3.5	1.3	−1.5*	1.3*
Invariant chain/X00496b	1.59	−2.2	−2.8*	1.2*

^aAsterisks indicate changes relative to IFN-γ alone that reached statistical significance in microarray studies (see Materials and Methods). RT-PCR studies indicated statistically significant inhibition by both 19-kDa lipoprotein and M. tuberculosis H37Ra of invariant chain induction by IFN-γ.

^bInvariant chain is included in this table, although it narrowly missed fulfilling the 1.6-fold criterion for induction by IFN-γ in microarray studies. Inhibition of invariant chain expression reached statistical significance with M. tuberculosis H37Ra and H37Rv but not 19-kDa lipoprotein. RT-PCR studies indicated statistically significant inhibition by both 19-kDa lipoprotein and M. tuberculosis (P < 0.001).