FIG. 1.

Increased expression of bfl-1 in urethral epithelium infected with live or gentamicin-killed N. gonorrhoeae. UECs received medium alone (uninfected) or were challenged for 4 h with live (1291) or gentamicin-killed (1291+Gent) gonococci. After infection, total RNA was harvested, and cDNA was synthesized. PCR analysis then demonstrated that expression of the antiapoptotic Bcl-2 family member bfl-1 is increased in UECs infected with either live or gentamicin-killed gonococci. Panel 1, 18S rRNA internal control; panel 2, bfl-1; panel 3, reverse transcriptase-negative controls.