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Indirect immunofluorescent detection of Stx binding and Gb3 on porcine peripheral blood leukocytes. Anti-CD77/Gb3 (A), Stx1 (B), and Stx2 (C) binding to cells on granulocyte smears are shown. Anti-CD77/Gb3 (E), Stx1 (F), and Stx2 (G) binding to cells on mononuclear leukocyte smears are also shown. (D and H) Differential interference contrast illumination of the same fields as those in panels C and G, respectively. Images were obtained as described in the legend to Fig. 1. Bar = 25 μm.
