FIG. 6.

Survival of Mma11 (♦) and Mma20 (░⃞) in the human macrophage cell line THP-1 (A) and the carp leukocyte cell line CLC (B). Bacteria and cells were incubated at 33°C for THP-1 and at 28°C for CLC. After a 1-h coincubation of cells and bacteria, the cells were washed, treated with 200 μg of amikacin per ml, and incubated in medium with 30 μg of amikacin per ml at the appropriate temperature. At different time points, samples were taken for CFU determination. Survival was expressed as the percentage of CFU at each time point, with the number of CFU at time zero as a reference. The means from three experiments are shown.