FIG. 5.

Pups born to immunized dams had significant levels of Pneumocystis-specific IgG in the serum and BALF. Dams were given i.t. inoculations of Pneumocystis on the day of breeding and boosted with i.n. inoculums on day 16 of gestation. Control dams received inoculations of PBS. Pups in both groups were given i.n. inoculations of Pneumocystis within 72 h of birth. ELISAs were performed on BALF and serum collected from pups in each group to determine the level Pneumocystis-specific (A, B) IgG or (C) IgA present. Data are expressed as the optical density at 405 nm. BALF samples were undiluted and serum samples were diluted 1:100 for the ELISA assay. Data represent the means ± SD for four to five mice per group. *, P < 0.05 compared to pups from naïve dams.