TABLE 2.
Comparison of innate and adaptive cell infiltrates to OD and CV tissues with differing inoculative doses
| Dose | OD
|
Ratio | CV
|
Ratio | ||
|---|---|---|---|---|---|---|
| No. of Innate cellsa | No. of Th1 cellsb | No. of Innate cellsa | No. of Th1 cellsb | |||
| 1 × 107 | 600 (74) | 40 (0.8) | 15 | 390 (83) | 20 (16) | 20 |
| 1.5 × 105 | 500 (73) | 36 (13) | 14 | 400 (23) | 39 (10) | 10 |
| 1 × 104 | 300 (61) | 37 (9.7) | 8.1 | 230 (38) | 18 (18) | 13 |
a
Number of PMN (103) and DC (103) per 106 GT cells (± standard errors of the means) 7 days postinfection. PMN and DC were identified in a pool of genital tract cells by flow cytometry, using LY6G and CD11c monoclonal antibodies, respectively. Data were compiled from three independent experiments.
b
Number of Th1 cells (103) per 106 GT cells (± standard errors of the means) 14 days postinfection. Chlamydia-responsive Th1 cells were identified in a pool of genital tract cells using IFN-γ and IL-4 intracellular cytokine staining following a 48-h stimulation with Chlamydia antigen. Data were compiled from two independent experiments.