TABLE 1.
Primers in the 5′- and 3′-flanking regions of the tpr genes
| Subfamily and gene | Sense primer (5′-flanking region) | Antisense primer (3′-flanking region) | Amplicon size (bp)a |
|---|---|---|---|
| Subfamily I | |||
| tprC | 5′-GGGGGTGAGGTAGAAGTGAGA | 5′-CCCTCATCCGAGACAAAAT | 1,916 |
| tprD | 5′-AAGAGGTTCAGGAAGCAACG | 5′-ACTTCGTAGGAGCAGCAGGA | 2,792 |
| Subfamily II | |||
| tprG1 | 5′-CAGGATTTTCCGGTTCATTG | 5′-TCACGCGTTTAATGTTCTGC | 3,257 |
| tprG2 | 5′-AAGTTTGCTTTCAGAT | 5′-GTACTACCTTCCCCCGGTCT | 3,018 |
| tprG/I hybrid | 5′-CGCGTACCCACTTCTCTCTC | 5′-GTACTACCTTCCCCCGGTCT | 2,459 |
| Subfamily III | |||
| tprA | 5′-CGTATGCTTTTACCCGCTGT | 5′-TGCAACCATCTTCGATTACG | 2,795 |
| tprB | 5′-CTTCGCGCCTAAGTTAATGC | 5′-TACTCATGCGGATCCCCTAC | 3,011 |
| tprK | 5′-TCCCCCAGTTGCAGCACTAT | 5′-TCGCGGTAGTCAACAATACCA | 2,083-2,133b |
| tprL | 5′-TTCTGAGCAGCTAGGCATTG | 5′-ACGGCGTCTTTCTGTACGTG | 1,876 |
| tprH | 5′-ACGTGGCAGAGGAGTTTTTG | 5′-TTCCTCAGGAGTTTGGAACG | 3,197 |
a
The size refers to amplicons obtained with T. paraluiscuniculi DNA as the template.
b
Size range of 10 tprK amplicons.