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. 2004 Nov;72(11):6237–6244. doi: 10.1128/IAI.72.11.6237-6244.2004

FIG. 5.

FIG. 5.

Identification of the SdrX ligand. (A) Screening of ECM proteins by a fluorescent bead-based assay. The rSdrX-A protein was covalently coupled to fluorescent beads and incubated with the immobilized human ECM proteins listed on the horizontal axis. Immobilized BSA served as a negative control. The vertical bars indicate the mean fluorescence and standard deviations measured in duplicate wells. (B) Bacterial adherence assay. S. capitis strain ATCC 35661 was bound to immobilized human collagen I, II, III, V, and VI and BSA. Symbols indicate the mean absorbance and standard deviations of duplicate wells. (C). Inhibition of binding of S. capitis strain ATCC 35661 to immobilized collagen VI. Bacteria were incubated with rabbit anti-rSdrX-A (diamonds) or normal rabbit IgG (squares). Symbols indicate the mean percent bound and standard deviations of duplicate wells.