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M. catarrhalis HumA was expressed on the surface of the complemented E. coli strain [K-12 EB53(pREP4) plus (pQE-P91E)] as shown by colony lift analysis with anti-HumA antibodies (A). E. coli K-12 EB53(pREP4) plus pQE-P91EMut was a negative control (B). Growth conditions were LB medium with kanamycin, ampicillin, IPTG, and ALA.
