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Phylogenetic conservation of TcG1-TcG8 in various T. cruzi strains. Genes TcG1-TcG8 were amplified in a PCR using gene-specific primers from genomic DNA of CL/Brenner (CL), SylvioX10/4 (S), Brazil (Br), and Y strains of T. cruzi. Plasmid containing the specific gene (P) was used as a template in gene-specific PCR to confirm the accuracy of the reaction. The GPI8 gene was amplified as a positive control. Shown are the amplicons resolved on 1% agarose gel.
