TABLE 1.

Oligonucleotides used in this study^a

Gene and use	Forward primer sequence (5′ → 3′)	Reverse primer sequence (5′ → 3′)	Size (bp)
Gene amplificationb
TcG1	CACCAGTTTCTGTACTATATTG	CGTTCGAGATGCGCTTCT	538
TcG2	CACCAGTTTCTGTACTATATTG	CCCAACAGCGGTGGAA	839
TcG3	CACCAGTTTCTGTACTATATTG	AATCCCCTGATACGTCG	1,518
TcG4	CACCAGTTTCTGTACTATATTG	TGACAGAACGTGAATGGG	600
TcG5	CACCAGTTTCTGTACTATATTG	TGAAGAAGAGCGTCGAGTGC	1,427
TcG6	CACCAGTTTCTGTACTATATTG	CACAGCAAGGGAGCAAC	799
TcG7	CACCAGTTTCTGTACTATATTG	CTTTTGCAATGGTCTTTGCG	636
TcG8	CACCAGTTTCTGTACTATATTG	TCACTGTGGTACAACGCTGACC	1,256
TcGP18	AAGCTTCGAGCATTGTCTATGTGCCTTGAA	CTCGAGCTACAGCAGGTCATATTGTACATC	450
Genomic conservation, expression analysis, and cloningc
TcG1	GGATCCATGGTGAAGGCGAACTATATT	GGGTCTAGATCACGTTCGAGATGCGCTTC	499
TcG2	GGATCCATGTCGCTTTCATTTATCGAGTCAGGG	GGGTCTAGATCACCCAACAGCGGTGGAA	662
TcG3	GGATCCATGCTTCAGCGTACCTGCAGC	GGGTCTAGATCAGCTTGACACTTCGC	1,011
TcG4	GGATCCATGTCAGCCAAGGCTCCC	GGGTCTAGATCACTTTTCAAGCGCC	276
TcG5	GGATCCATGGGGAAGGAAAAGGTGC	GGGTCTAGATCACTTCTTAGCGGC	1,350
TcG6	AAGGCTATGCTGGCGACAC	GGGTCTAGATCACACAGCAAGGG	756
TcG7	GGATCCATGCTGGCGACACACGG	GGGTCTAGACTACATCCATCCTCGCC	351
TcG8	GGATCCATGTCCGATAACCATCAACTGG	GGGTCTAGATCACTGTGGTACAACGCTG	1,188
TcGP18	AAGCTTCGAGCATTGTCTATGTGCCTTGAA	CTCGAGCTACAGCAGGTCATATTGTACATC	450

^aUnderlined sequences represent restriction sites.

^bPrimers were used for gene amplification by traditional RT-PCR.

^cPrimers were used to test genomic conservation (Fig. 2), for expression analysis (Fig. 3), and for cloning the genes in genetic immunization constructs.