TABLE 3.

T. cruzi sequences exhibiting characteristics of membrane-associated or secreted proteins^a

NCBI accession no.	Selected ORF	PSORT localization (P value)	N-terminal signal sequence P value	GPI C/A site	GPI addition signal sequence
AI0810133	5′-3′ frame 2	PM (0.6)	0.75	+	+
AQ910890	5′-3′ frame 1	PM (0.6)	1.0	+	−
AW325178	3′-5′ frame 2	PM (0.4)	1.0	−	+
AW325227	5′-3′ frame 2	PM (0.6)	0.75	+	−
AW325291	3′-5′ frame 3	PM (0.6)	0.25	+	−
AW329906	5′-3′ frame 3	OS (0.3)	1.0	+	−
AW330105	5′-3′ frame 3	PM (0.4)	1.0	+	−
AW330110	5′-3′ frame 3	PM (0.6)	1.0	+	−
AW330132	3′-5′ frame 2	PM (0.6)	0.75	+	−
AW330166	3′-5′ frame 1	PM (0.9)	1.0	+	+
AW330173	5′-3′ frame 2	PM (0.3)	0.5	+	−
AW330187	5′-3′ frame 1	OS (0.8)	0.75	+	−
AW330209	5′-3′ frame 3	PM (0.4)	0.5	+	+
AW330228	5′-3′ frame 2	PM (0.7)	0.75	+	+
AW330247	3′-5′ frame 3	PM (0.4)	0.75	+	+
AW330324	5′-3′ frame 1	OS (0.8)	1.0	−	+
AW330353	3′-5′ frame 1	PM (0.6)	1.0	+	+
AW330404	3′-5′ frame 2	PM (0.6)	0.0	+	+
AZ050159	3′-5′ frame 1	OS (0.8)	1.0	+	−

^aAs predicted by computational and bioinformatic analysis of EST and GSS databases. Localization of the encoded products to plasma membrane (PM) and outside (OS) was determined by PSORT analysis (probability score, 0 to 1). The presence of N-terminal signal sequence was assessed by SignalP (probability score, 0 to 1), the score of 1 being considered maximum for both PSORT and SignalP. The GPI cleavage-attachment (C/A) site of three amino acids (ω, ω + 1, ω + 2) and a GPI addition signal sequence in the C-terminal region of the ORF were predicted by DGPI. “+” and “−” signs represent the probability of the presence or absence, respectively, of a GPI C/A site or GPI addition signal.