FIG. 2.

To investigate the localization of PilA in the wild-type and mutant strains, whole bacterial lysates (WCL; odd lanes) or sheared surface components (SS; even lanes) were analyzed by SDS-PAGE and immunoblotting. Comparative analysis of the MAb 4G9-probed immunoblot indicated that whereas the wild-type 7169 contained pilin subunits both within the cell and on the surface (lanes 1 and 2), pilAK4 exhibited the expected PilA-null phenotype (lanes 3 and 4), pilTK3 appeared to have slightly more pilin in the SS (lanes 5 and 6), and pilQK6 contained pilin subunits within the cell but not on the cell surface (lanes 7 and 8). Molecular size standards are shown in kilodaltons.