FIG. 2.

Kinetics of K^bS-specific CD8⁺-T-cell response to pCI/S immunization in different organs. Mice were immunized with plasmid pCI/S, and the mean frequencies of IFN-γ-responding K^bS-specific CD8⁺ T cells were determined, as described in Materials and Methods, for multiple time points. The graph shows this response in the spleen (•), liver (○), and draining (inguinal) LN (▴). Data were obtained from 62 pCI/S-immunized mice in seven independent experiments. n = 3 to 6 for each time point for each organ.