FIG. 5.

Polyclonal CD8⁺ memory T-cell responses to Salmonella. Single-cell suspensions of organs from Salmonella-immunized animals were restimulated either with medium alone or with Salmonella lysate and were assessed for IFN-γ and TNF-α expression. Plots represent the CD8⁺-T-cell responses of individual mice who were immunized 28 weeks previously with Salmonella. Data are representative of at least six individuals for each organ. Similar results were obtained in two to four independent experiments. Plots show gated viable TCRβ⁺ CD8⁺ cells. All axes represent log fluorescence intensities. Staining with appropriate isotype control MAbs did not result in cells within the indicated gate (data not shown).