TABLE 2.

Effect of neutralizing IL-12 on T-cell cytokine production by splenocytes from Salmonella-immunized mice after restimulation with Salmonella lysate^a

Mouse no.	% CD4 IFN-γ-producing cells		% CD8 IFN-γ-producing cells		% CD4 TNF-α-producing cells		% CD8 TNF-α-producing cells
	Isotype Ab	Anti-IL-12	Isotype Ab	Anti-IL-12	Isotype Ab	Anti-IL-12	Isotype Ab	Anti-IL-12
1	2.0	1.2	8.4	6.1	0.66	0.62	0.14	0.20
2	1.4	0.89	7.3	5.8	0.31	0.39	0.005	0.017
3	1.7	1.4	2.7	2.4	0.6	0.83	0.11	0.14
4	1.0	1.1	4.4	4.1	0.36	0.38	0.001	0.012
5	8.3	9.2	16	7.7	2.7	4.1	0.154	0.062

^aSplenocytes from mice orally infected with Salmonella 26 weeks earlier were stimulated with Salmonella lysate in the presence of either a neutralizing antibody to IL-12 (clone C17.8) or a rat IgG2a isotype control, as indicated. The numbers show the percentages of cytokine-producing cells after the percentages of cells staining with the isotype-matched control antibody were subtracted. The data are from five infected mice.