Table 2. The mean (± S.E.) growth conditions of Trichodesmium erythraeum IMS101 cultures for the temperature and light response.
| Temperature response | Light response | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Low CO2 | Mid CO2 | High CO2 | Low CO2 | Mid CO2 | High CO2 | |||||
| Variables | Units | LL | HL | LL | HL | LL | HL | |||
| pH | Total | 8.506 | 8.477 | 8.171 | 8.175 | 7.901 | 7.908 | 8.524 | 8.214 | 7.957 |
| H+ | nM | 3.1(±0.02) | 3.3(±0.032) | 6.7(±0.044) | 6.7(±0.041) | 12.6(±0.074) | 12.3(±0.056) | 3.0(±0.016) | 6.1(±0.033) | 11.0(±0.074) |
| AT | μM | 3275(±22) | 2993(±25) | 2723(±21) | 2796(±16) | 2549(±17) | 2626(±11) | 3004(±6) | 2963(±9) | 2936(±93) |
| TCO2 | μM | 2387(±23) | 2187(±18) | 2242(±19) | 2298(±14) | 2264(±15) | 2332(±10) | 2146(±5) | 2410(±7) | 2583(±14) |
| HCO3- | μM | 1765(±23) | 1639(±15) | 1922(±17) | 1962(±12) | 2067(±13) | 2127(±9) | 1562(±5) | 2031(±5) | 2330(±12) |
| CO32- | μM | 618(±5) | 544(±9) | 313(±4) | 326(±4) | 178(±3) | 186(±2) | 580(±2) | 370(±2) | 235(±2) |
| CO2 | μM | 3.89(±0.09) | 3.85(±0.08) | 9.14(±0.11) | 9.17(± 0.10) | 18.41(±0.18) | 18.64(±0.15) | 3.25(±0.03) | 8.62(±0.05) | 17.86(±0.06) |
| pCO2 | ppm | 139(±2) | 138(±2) | 325(±3) | 330(±2) | 649(±3) | 658(±2) | 118(±1) | 312(±2) | 647(±2) |
| Chl a | μg L-1 | 15.9(±1.1) | 16.2(±1.0) | 23.0(±1.7) | 19.6(±1.5) | 25.4(±1.8) | 20.6(±1.7) | 21.8(±1.3) | 28.8(±1.6) | 36.3(±2.1) |
| n | 46 | 63 | 74 | 78 | 83 | 98 | 107 | 124 | 102 | |
For the temperature response, the growth conditions per CO2 and light treatment is the average of all temperature treatments. For the light response, the growth conditions per CO2 treatment is the average of all light treatments. The total inorganic carbon (TCO2), bicarbonate (HCO3-), carbonate (CO32-) and pCO2 was calculated via CO2SYS using the pH and AT concentration. Temperature response growth conditions; low (180 ppm), mid (380 ppm) and high (720 ppm) CO2, 40 μmol photons m-2 s-1 (LL) and 400 μmol photons m-2 s-1 (HL), ranging between 18–31°C. Light response growth conditions; low (180 ppm), mid (380 ppm) and high (720 ppm) CO2, 26°C, ranging between 20–1400 μmol photons m-2 s-1)