Fig 1. Partial sequence alignment of β-tubulin isotype 1 gene for Necator americanus, Ascaris lumbricoides and Trichuris trichiura.

SNP 167T>A, SNP 198A>C, and SNP 200T>A SmartAmp2 primer sets were designed with the 3’-end or 5’-end of one or two primers are overlapping inter-species nucleotide variations (species-specific).