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. 2017 Jan 12;11(1):e0005205. doi: 10.1371/journal.pntd.0005205

Fig 2. SmartAmp2 primer design for β-tubulin SNP detection in Ascaris lumbricoides.

Fig 2

Partial sequence of the β-tubulin isotype 1 gene carrying (A) SNP 167A, (B) SNP 198C, and (C) SNP 200A as well as the sequences of primers used for the SmartAmp2 assay for the three SNP positions. The locations of SNPs indicated in bold. Both TP (turn-back primer) and FP (folding primer) were used as discrimination primers to target F167Y (TTC>TAC) SNP. BP (boost primer) was used as discrimination primer to target E198A (GAA>GCA) and F200Y (TTC>TAC) SNPs. The folding primer (FP) has a specific sequence (CCTATATATATATAGG) at the 5’ end to allow self-annealing hairpin formation.