Table 1. List of primers and probes used in this study.

Name	Length	Sequences a	Position b
Probe LN34	17	(FAM) AA+C+ACCY+C+T+ACA+A+TGGA (BHQ1)	59–75
Probe LN34a	17	(FAM) AAC+ACCYC+T+ACA+A+TGGA (BHQ1)	59–75
Probe LN34lago	17	(FAM) AA +C +ACTA +C +T +ACA +A +TGGA (BHQ1)	59–75
Probe LN34m	17	(6FAM)-ACACCYCTACAATGGAT-(MGBNFQ)	60–76
Primer forward1	24	ACGCTTAACAACCAGATCAAAGAA	1–24
Primer forward2	25	ACGCTTAACAACAAAATCADAGAAG	1–25
Primer reverse	25	CMGGGTAYTTRTAYTCATAYTGRTC	140–164

^a The probes are labeled by fluorescent FAM at the 5′end, Black Hole quencher (BHQ1) at the 3′ end except probe LN34m which is labeled by MGB and NFQ quencher. LNA modified bases are indicated by a plus preceding the base in the sequence (e.g. +A, +G, +C, +T).

^b The primer and probe positions are given relative the Lyssavirus full genome (see below).