Fig 2. Effect of haemoglobin on promastigote growth.

Promastigotes were cultured in triplicate in three media differing in haemoglobin content; M1 (0.0099 g/L), M2 (0.495 g/L) and M3 (0.99 g/L). These media were accompanied by a negative control medium containing no haemoglobin (M0). Promastigote growth seems related to haemoglobin concentration, with the most rigorous growth and highest cell densities observed in M3; the media with the highest haemoglobin concentration. The slowest growth and lowest cell densities were observed in M0, the negative control.