Table 1. Bacterial strains used in this study.
| Strains and plasmids | Genotype or descriptiona | Source |
|---|---|---|
| S. aureus | ||
| COL | Clinical MRSA expressing pls | [27] |
| COLsdgA/sdgB | Deficient in sdgA/sdgB (Kanr) | This study |
| 1061 | Clinical MRSA expressing pls | [28] |
| 1061pls | Deficient in pls (Tcr) | [26] |
| 1061pls (pPLS4) | Complemented pls mutant (Tcr Cmr) | [26] |
| 1061pls (pPLS6) | Deletion of SD repeats of Pls (Tcr Cmr) | [29] |
| 1061pls (pPLSsub1) | Production of Pls with 17 aa of SD repeats (Tcr Cmr) | This study |
| 1061pls (pPLSsub2) | Production of Pls with 34 aa of SD repeats (Tcr Cmr) | This study |
| 1061pls (pPLSsub3) | Production of Pls with 130 aa of SD repeats (Tcr Cmr) | This study |
| SA113 | ATCC 35556, NCTC 8325 derivative, rsbU- | [30] |
| SA113 (pCU1) | Empty vector control (Cmr) | This study |
| SA113 (pPLS4) | Expression of pls (Cmr) | This study |
| SA113gtfA (pPLS4) | Deficient in gtfA, expression of pls (Cmr) | This study |
| SA113bgt (pPLS4) | Deficient in the putative bgt, expression of pls (Cmr) | This study |
| SA113gtfA/sdgA/sdgB (pPLS4) | Deficient in gtfA, sdgA, sdgB, expression of pls (Emr Cmr) | This study |
| SA113sdgA/sdgB | Deficient in sdgA, sdgB (Emr) | This study |
| SA113sdgA/sdgB (pCU1) | Empty vector control (Emr Cmr) | This study |
| SA113sdgA/sdgB (pPLS4) | Expression of pls (Emr Cmr) | This study |
| SA113sdgA/sdgB (pPlsGtfCDCOL) | Expression of pls, gtfC, gtfD from strain COL (Emr Cmr) | This study |
| SA113sdgA/sdgB (pPlsGtfΔCDCOL) | Expression of pls, ΔgtfC, gtfD from strain COL (Emr Cmr) | This study |
| SA113sdgA/sdgB (pPlsGtfCΔDCOL) | Expression of pls, gtfC, ΔgtfD from strain COL (Emr Cmr) | This study |
| SA113sdgA/sdgB (pPlsGtfΔCΔDCOL) | Expression of pls, ΔgtfC, ΔgtfD from strain COL (Emr Cmr) | This study |
| SA113sdgA/sdgB (pPlsGtfCD1061) | Expression of pls, gtfC, gtfD from strain 1061 (Emr Cmr) | This study |
| SA113sdgA/sdgB (pPlsGtfΔCD1061) | Expression of pls, ΔgtfC, gtfD from strain 1061 (Emr Cmr) | This study |
| SA113sdgA/sdgB (pPlsGtfCΔD1061) | Expression of pls, gtfC, ΔgtfD from strain 1061 (Emr Cmr) | This study |
| SA113sdgA/sdgB (pPlsGtfΔCΔD1061) | Expression of pls, ΔgtfC, ΔgtfD from strain 1061 (Emr Cmr) | This study |
| SH1000 | ATCC 35556, NCTC 8325 derivative, rsbU+ | [31] |
| SH1000 (pPLS4) | Expression of pls (Cmr) | This study |
| SH1000sdgA/sdgB | Deficient in sdgA/sdgB (Kanr) | This study |
| SH1000sdgA/sdgB (pPLS4) | Expression of pls (Kanr Cmr) | This study |
| Newman | NCTC 8178, clinical isolate | [32] |
| Newman (pPLS4) | Expression of pls (Cmr) | This study |
| Newman (pCU1) | Empty vector control (Emr Cmr) | This study |
| Newman (pPlsGtfCDCOL) | Expression of pls, gtfC, gtfD from strain COL (Emr Cmr) | This study |
| Newman (pPlsGtfΔCΔDCOL) | Expression of pls, ΔgtfC, ΔgtfD from strain COL (Emr Cmr) | This study |
| Newman (pΔPlsGtfCDCOL) | Expression of Δpls, gtfC, gtfD from strain COL (Emr Cmr) | This study |
| NewmanC | Newman (pPlsGtfCDCOL) cured from its plasmid | This study |
| NewmanC (pCU1) | NewmanC transformed with plasmid pCU1 | This study |
| NewmansdgA/sdgB | Deficient in sdgA/sdgB (Kanr) | This study |
| NewmansdgA/sdgB (pPLS4) | Expression of pls (Kanr Cmr) | This study |
| NewmansdgA/sdgB (pCU1) | Empty vector control (Emr Cmr) | This study |
| NewmansdgA/sdgB (pPlsGtfCDCOL) | Expression of pls, gtfC, gtfD from strain COL (Emr Cmr) | This study |
| NewmansdgA/sdgB (pPlsGtfΔCΔDCOL) | Expression of pls, ΔgtfC, ΔgtfD from strain COL (Emr Cmr) | This study |
| NewmansdgA/sdgB (pΔPlsGtfCDCOL) | Expression of Δpls, gtfC, gtfD from strain COL (Emr Cmr) | This study |
| NewmansdgA/sdgBC | NewmansdgA/sdgB (pPlsGtfCDCOL) cured from its plasmid | This study |
| NewmansdgA/sdgBC (pCU1) | NewmansdgA/sdgBC transformed with plasmid pCU1 | This study |
| Cowan 1 | ATCC 12598, NCTC 8530, isolate from septic arthritis | [32] |
| S. carnosus | ||
| TM300 | Non-pathogenic reference isolate | [33] |
| TM300 (pCU1) | Empty vector control (Cmr) | This study |
| TM300 (pPlsGtfCDCOL) | Expression of pls, gtfC, gtfD from strain COL (Emr Cmr) | This study |
| TM300 (pPlsGtfΔCDCOL) | Expression of pls, ΔgtfC, gtfD from strain COL (Emr Cmr) | This study |
| TM300 (pPlsGtfCΔDCOL) | Expression of pls, gtfC, ΔgtfD from strain COL (Emr Cmr) | This study |
| TM300 (pPlsGtfΔCΔDCOL) | Expression of pls, ΔgtfC, ΔgtfD from strain COL (Emr Cmr) | This study |
| TM300 (pPlsGtfCD1061) | Expression of pls, gtfC, gtfD from strain 1061 (Emr Cmr) | This study |
| TM300 (pPlsGtfΔCD1061) | Expression of pls, ΔgtfC, gtfD from strain 1061 (Emr Cmr) | This study |
| TM300 (pPlsGtfCΔD1061) | Expression of pls, gtfC, ΔgtfD from strain 1061 (Emr Cmr) | This study |
| TM300 (pPlsGtfΔCΔD1061) | Expression of pls, ΔgtfC, ΔgtfD from strain 1061 (Emr Cmr) | This study |
| S. epidermidis | ||
| RP62A | Strong biofilm producer | [34] |
| E. coli | ||
| XL1-Blue | supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac F’[proAB+ lacIq lacZΔM15 Tn10] (Tcr), cloning host | [35] |
aMRSA, methicillin-resistant S. aureus; gtf, glycosyltransferase; bgt, bactoprenol glycosyltransferase, pls, plasmin-sensitive protein; Tcr, tetracycline resistant; Cmr, chloramphenicol resistant; Emr, erythromycin resistant; Kanr, kanamycin resistant.