Table 3. Primers used in this study.
| Primer name | Oligonucleotide sequence (5´→ 3´; restriction site underlined) |
|---|---|
| TE-P1-PstI1 | AACTGCAGCCTAAAATGTAATTCATATTATCGCCTC |
| TE-P3-HindIII1 | CCCAAGCTTGGTTTAACAACAACAGGTGTTATTAAAGATGC |
| TE-P1-XbaI1 | GAGTTATACAACTCTAGAGAGGTATAATAAAAACGCGC |
| TE-P4-EcoRI1 | GGACATATCTTAGAATTCTTAACGGAGGAAAAAAATGACTGAATTTGATTATCG |
| Rmgts41 | TATATGAATTCATACACCTGTTAAACCAATGAGTAC |
| Rmgts51 | TTATAGGTACCGGCATTGCTTTTCTGTTGATAC |
| Rmgts61 | TTTAAGGTACCTACTGTGAAGCACAGCTACTAC |
| Rmgts71 | TATTAGGATCCGTACTTGTTGACCAGTATCAAAC |
| Fkan11 | GGCGGGGTACCCAGCGAACCATTTGAGG |
| Rkan21 | GGGGCGGTACCAATTCCTCGTAGGCGCTCGG |
| PlsGtfCD-F1 | ATATGGTACCGGTATAGGGGGAGCCATTGT |
| PlsGtfCD-R1 | ATATGGTACCGGATGATGCTTTAAAGAGTGTCG |
| Pls4Sub1-R1 | GTCTGCATCAGAATCGCTATCTGCGTCTGAATCGCTGTCCGC |
| Pls4Sub2-R1 | GCTGTCTGAATCGCTGTCCGCATCAGAGTCGCTATCTGCGTC |
| Pls4Sub3-R1 | TGCGTCTGAGTCGCTGTCTGCATCAGAATCGCTATCTGC |
| Pls4Sub1/2/3-F1 | AGAGATCATAATGACAAAACAGATAAACCAAATAATAAAGAG |
| GtfC-F2 | GTAGTACTAATTTCTTACAAAATATG |
| GtfC-R2 | CAATAAGTGAGTTGTCTCATATG |
| GtfD-F2 | CAGGAGAATAACGTGCAACGG |
| GtfD-R2 | GATCTATATGATTCAAGAGGCT |
| GtfC-mutF2 | TGGCAGACGTCGTAAAAACA |
| GtfC-mutR2 | TCAAGCACTCTAAAGCTTTTTCAA |
| GtfD-mutF2 | AAAAACCCATCAAGAATACTAGGAA |
| GtfD-mutR2 | GAACGACAAAACTTCACTGTTGA |
| Pls6B-F2 | CGCGGATCCTACCAATGAATATGGTTGTTACAAATAG |
| Pls6K-F-15812 | TATGGTACCGCAGATAGAGATCATAATGACAAAACAGATAAACC |
| Pls6B-R-16372 | ATAGGATCCTTATTTTTCTTCATTATTTTTGTTTTTACGACGTCTGCC |
| Sub-SD-R2 | ATCATCTTTAGCACCATGGATGATTACTTCATC |
| Pls-F2 | GGTAATGTTCAAACTATTGAAC |
| Stop-Pls-R2 | TTATTTTTCTTCATTATTTTTGTTTTTA |
| SdgAB-B-F2 | ATATGGATCCAACGGCTCAAATAACGCAACG |
| SdgAB-X-R2 | ATATCCCGGGATCGACACGAGAAGGTCGTT |
| SdgA-F22 | TCGTCCTCATGAATTAGGAAATG |
| SdgB-R22 | AACGTCCTGATGAAAAACGTG |
| 1061-SdgB-F2 | CAAATTGGTCCCATTGTTTAAT |
| 1061-IS1181-R2 | TGAACGATATGGAATCTGTCAAA |
| SaeS-F2 | CCGTATTAGAGAAAAATTAGAAAAAGAGAGC |
| SaeS-R2 | CAAAAAAAGAAGCCCTCATTAATGGG |
| SaeR-F2 | GAGTCACTCATTGTTAAAACAGATTTCAC |
| SaeR-R2 | ATGCAATTGCTAAAATAGTTGAAGTTAATGG |
Primers used for 1cloning and 2sequencing.