Fig 4. Induction of lipid synthesis by adiponectin in human sebocytes by activating APPL1-AMP activated protein kinase (AMPK) signaling.

(A) After sebocytes were treated with adiponectin, whole cell lysates were prepared and analyzed by Western blotting. Blots were incubated with specific antibodies. Densitometric analyses of these protein signals were normalized relative to those for actin controls. (B) Sebocytes were transfected with control, AdipoR1 or AdipoR2-targeting siRNA and the intracellular protein levels of each signals were determined by Western blotting. (C) Sebocytes were incubated with different concentrations of compound C and protein signals were determined by Western blotting and intracellular lipid levels were measured by Oil Red O staining. Data represent means ± SEM (n = 6). Data were analyzed by Student’s t test (*P < 0.05, **P < 0.01, ***P < 0.001 vs. untreated cells; †P < 0.05, ‡P < 0.01 vs. adiponectin-treated cells).