Fig. 2.
Laser induced calcium responses of HL-1 cells in HBSS + Ca2+ after laser stimulation. An orthogonal projection (time plotted along the green lines) of Visualization 1 (7.3MB, MP4) shows different effects of the laser stimulation on calcium signaling with a radiant exposure of 27 mJ/cm2 in HL-1 cells (a). In both projections, a calcium shock can be observed over time for the cell, which is in the laser treated area after laser application. Calcium oscillations are visible at the periphery or outside of the laser spot. Scale bar 50 µm. Panels b) and c) show the quantified response of a single representative cell. Laser irradiation occurred at 0 s (arrow pointing to asterisk) with the laser spot being located within the marked area. Cells within the laser spot mostly responded with a calcium shock, while cells not directly irradiated often exhibited calcium oscillations after laser treatment. Please refer to Visualization 1 (7.3MB, MP4) for a time series of subfigures a) to c).