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. 2017 Jan 12;12(1):e0168409. doi: 10.1371/journal.pone.0168409

Fig 1. A61603 potently activates ERK in cultured neonatal rat ventricular myocytes (NRVMs).

Fig 1

ERK activation was quantified by immunoblot as the ratio of phospho- to total-ERK (P-ERK/T-ERK) after 10 min with the beta-blocker propranolol (PROP) 200 nM, then 5 min with various α1-agonists or vehicle. Dose-response curves normalized to vehicle+PROP were analyzed by non-linear regression (N = 3–8 independent cultures, each dose in duplicate in each experiment). A61603 blots are shown. Table 1 has Emax and EC50 for each agonist.