Table 2. Echocardiography of male mice treated with DOX.

	WILD TYPE				Alpha-1A KO
	Pre-DOX	Post-DOX			Pre-DOX	Post-DOX
		Vehicle	A61603	A6/Veh		Vehicle	A61603	A6/Veh
mice (n)	13	7	6		12	4	8
FS (%)	58 ± 4	49 ± 3 (12)++	61 ± 2 (14)	1.20***	58 ± 1	38 ± 4+++	38 ± 4+++	0.99
LVIDd (mm)	3.1 ± 0.1	2.5 ± 0.1+++	2.7 ± 0.1++	1.08	3.0 ± 0.1	2.3 ± 0.3+	2.3 ± 0.2++	0.98
LVIDs (mm)	1.3 ± 0.1	1.4 ± 0.1	1.3 ± 0.1	0.89	1.2 ± 0.1	1.4 ± 0.1	1.4 ± 0.2	1.01
LVPWd (mm)	0.9 ± 0.0	1.0 ± 0.1	0.9 ± 0.1	0.92	0.9 ± 0.0	1.1 ± 0.1	1.1 ± 0.1	1.02
LVPWs (mm)	1.6 ± 0.1	1.2 ± 0.1++	1.4 ± 0.1	1.15	1.5 ± 0.0	1.1 ± 0.1++	1.3 ± 0.1++	1.12
IVSd (mm)	0.9 ± 0.1	1.00 ± 0.1	0.9 ± 0.1	0.95	0.9 ± 0.0	0.9 ± 0.1	1.0 ± 0.1	1.05
IVSs (mm)	1.7 ± 0.1	1.4 ± 0.1	1.6 ± 0.1	1.11	1.7 ± 0.0	1.4 ± 0.2+	1.4 ± 0.1+	1.06
SV (μV (±l)	34 ± 1	17 ± 2+++	23 ± 3+++	1.37*	31 ± 2	15 ± 1++	14 ± 3+++	0.74
HR (bpm)	663 ± 12	300 ± 79+++	513 ± 77+	1.71*	657 ± 11	252 ± 16+++	214 ± 47+++	0.85
CO (ml/min)	22 ± 1	6 ± 2+++	13 ± 2+++	2.27**	20 ± 1	4 ± 2+++	3 ± 1+++	0.79
CO/BW (ml/min/g)	0.8 ± 0.0	0.2 ± 0.1+++	0.7 ± 0.1++	3.04***	0.8 ± 0.0	0.2 ± 0.1+++	0.1 ± 0.0+++	0.59

WT or alpha-1A-KO mice average age 16.5 weeks had DOX 25 mg/kg i.p., then were treated with A61603 10 ng/kg/d or Vehicle by mini-pump.

2D-guided M-mode echo was done Pre-DOX in some mice, and Post-DOX in all mice, either at euthanasia for HF or at 7 days.

Values are mean ± SE, with n (mice) given or in parentheses.

The n for Post-DOX FS is higher, since this was the only value recorded in an initial series of mice.

p by ANOVA with Newman-Keuls multiple comparisons test: A61603 vs. Vehicle

*** = <0.001

** = <0.01

* = <0.05

Post-DOX vs. Pre-DOX

+++ = <0.001

++ = <0.01

+ = <0.05.

Pre-DOX values for WT vs. alpha-1A-KO did not differ (p>0.1).