Table 4. Primers pair sequences for real-time PCR.
Gene | Forward (5’-3’) | Reverse (5’-3’) | Reference |
---|---|---|---|
fas | ACTCCTATGTGGCAGCATAGAC | GTTTCAGCCTCAGACTCTTTGCC | JX456351 |
g6pd | TATCTTTGCCGAACGCTGTC | TGTCTCCTCTGGGCTGAAGT | XM010731711 |
atgl | CCATGCATCCGTCCTTCAACC | GAGATCCCTAACCGCCCACT | Yan et al. [37] |
cpt-1 | GCTGAGCCTGGTGAAGATGTTC | TCCATTTGGTTGAATTGTTTACTGTCC | Yan et al. [37] |
lpl | GCGGAAACACAGACCTTCAT | AGTCGGCACACGCTCATAG | JQ327827 |
dgat-2 | TTCGGTGCTTTCTGCAACTTCG | AAGGATGGGGAAGCGGAAGT | Yan et al. [37] |
β-actin | CTACGAGGGTTAGCCCTGCC | TGAAGGAGTAACCGCGCTCTGT | GU584189 |
lpl: lipoprotein lipase;
atgl: adipose triglyceride lipase;
cpt-1: carnitine palmitoyltransferase-1;
dgat-2: acyl CoA diacylglycerol acyltransferase 2;
fas: fatty acid synthase;
g6pd: glucose 6-phosphate dehydrogenase