Table 6. Selected Antimicrobial Resistance MIC Results and Resistance Genes Identified from Whole Genome Sequence Data.
| Antibiotic (μg/ml) | Isolate Id c | Relevant Resistance Genes Identifiedd | |||
|---|---|---|---|---|---|
| 1 | 13 | 12 | ATCC 29212 | ||
| Bacitracin | 8 | 16 | >128 | 16 | bcrA, bcrB, bcrR (isolate 12) |
| Chloramphenicol | 64 | 64 | 32 | 4 | cat (all isolates) |
| Ciprofloxacin | >16 | >16 | 1 | 0.5 | parC (S80I) and gyrA (S83I) mutations (isolates 1 and 13) |
| Enrofloxacin | >16 | >16 | 1 | 0.25 | parC (S80I) and gyrA (S83I) mutations (isolates 1 and 13) |
| Erythromycin | >64 | 0.25 | >64 | 1 | ermB (isolates 1 and 12), lsa(A) (all isolates and 29212) |
| Gentamicina | >2048 | 16 | 32 | 16 | aac(6’)-aph(2”) (isolate 1) |
| Neomycina | 128 | 128 | >2048 | 64 | aph(3’)-III (isolate 12) |
| Streptomycina | >2048 | >2048 | >2048 | 64 | str (isolates 1 and 13), aph(3’)-III and ant(6)-Ia (isolate 12) |
| Tetracycline | 128 | 128 | 64 | 32 | tetM (all isolates and 29212), tetS (isolates 1 and 13), tetL (isolate 12) |
| Trimethoprim-sulfamethoxazoleb | >32 | 0.064 | 0.094 | 0.047 | dfrG (isolate 1) |
| Sequence Type | 4 | 4 | 55 | 30 | |
a High-level aminoglycoside resistance (HLAR) is indicated by boxes
b MIC determined by Etest
c Isolate resistance, aside from known intrinsic resistance and HLAR, is indicated by red shading with bold text; isolate MIC values exceeding ATCC 29212 are indicated in blue shading with bold text.
d Assembled genomes were uploaded to ResFinder with a 98% threshold for gene identification and a minimum length of 60%. The PATRIC specialty gene finder tool was used to identify bcrABR genes. Multiple sequence alignment was performed in PATRIC to identify amino acid polymorphisms conferring fluoroquinolone resistance.