(A) Schematic illustration of the hippocampal recording configuration and whisker stimulation in anesthetized mice. (B) Representative LFP recordings and corresponding analysis of theta-phase and gamma-amplitude relation for control littermate (left) and GB1-cKO (right) mice. The raw signals (black) were high-pass filtered (1st row; grey and red, respectively) and then computed to extract the theta phase (second row) and gamma envelope (third row; grey and red, respectively) for control and GB1-cKO mice. (C) Normalized LFP power spectrum analysis for theta (4–8 Hz) and gamma frequencies (30–50 Hz, low gamma; 70–90 Hz, high gamma) in control littermate (n = 6) and GB1-cKO mice (n = 6) after whisker stimulation. Inset, Relative power changes for GB1-cKO and control littermate mice (theta band, p=0.007; unpaired t test; low gamma, p=0.042; Wilcoxon rank-sum test; and high gamma oscillations, p=0.738; Wilcoxon rank-sum test). (D) Gamma-amplitude modulation by theta-phase for wild-type (left) and GB1-cKO mice (right), before (control) and after whisker stimulation (post stim). Note the enhancement of theta-gamma coupling in wild-type after stimulus that does not appeared in GB1-cKO mice. (E) Normalized Phase-Lock Value (PLV), either in control and after stimulation for wild-type (p=0.017; paired t test), and GB1-cKO mice (p=0.62; paired t test). See also Figure 7—figure supplement 1. *p<.05; **p<0.01. .
DOI:
http://dx.doi.org/10.7554/eLife.20362.013