Figure 1. Symbiont-mediated differential expression of odorant binding protein 6 in tsetse larvae.

(A) Number of genes exhibiting significant differential expression, and a relative transcript abundance [in transcripts per million (TPM)] over 3, in Gmm^WT compared to Gmm^Apo larvae. Significance is based on a Baggerly’s test followed by a false detection rate correction (p<0.01). (B) Significantly enriched gene ontology categories, determined using a Fisher’s exact test. (C) Genes exhibiting significant differential expression (measure as fold-change in gene expression) in Gmm^WT compared to Gmm^Apo larvae, and a minimum TPM value of 1000. Significance was determined as in (A). (D) Enrichment analysis of odorant binding protein-encoding genes expressed in Gmm^WT adult males (purple) and females (yellow), and Gmm^WT larvae (green). (E) Relative expression (TPM) of tsetse odorant binding protein-encoding genes in Gmm^WT larvae, and their differential expression (measure as fold-change in gene expression) in Gmm^WT compared to Gmm^Apo larvae. Significance was determined as in (A). (F) Relative obp6 expression in Gmm^WT larvae, as well as larvae derived from symbiont-cured moms fed a diet supplemented with yeast and Wigglesworthia-containing bacteriome extracts (Gmm^bact/Wgm+), Wigglesworthia-free bacteriome extracts (Gmm^bact/Wgm-), Sodalis cell extracts (Gmm^Sgm+), and bacteriome extracts harvested from Gmm^Apo females (Gmm^bact/Apo). Gmm^WT and Gmm^Apo flies served as controls. n = 6 biological replicates for groups Gmm^WT, Gmm^bact/Wgm+ and Gmm^Sgm+ samples, and n = 5 biological replicates for Gmm^bact/Wgm-, Gmm^bact/Apo and Gmm^Apo samples. Replicates for all groups contain a mixture of four first and second instar larvae. Data are presented as mean ± SEM. Bars with different letters indicate a statistically significant difference (specific p values are listed in Figure 1—source data 1) between samples. Statistical analysis = ANOVA followed by Tukey’s HSD post-hoc analysis.

DOI: http://dx.doi.org/10.7554/eLife.19535.003

Figure 1—figure supplement 1. Developmental stage-specific enrichment analysis of tsetse orthologues that putatively cluster within the ‘hematopoiesis’ COG. .

Ontology enrichment analyses were performed as described in the Materials and methods, under the ‘Transcriptomics’ sub-heading. Refer to Supplementary file 2 for a description of the enriched hematopoiesis-associated gene expressed specifically in Gmm^WT and Gmm^Apo tsetse larvae.

DOI: http://dx.doi.org/10.7554/eLife.19535.005