Fig. 2. Triazole 1.1 does not affect ambulatory behaviors in mice.
(A) U50,488H (left) dose-dependently decreased locomotor activity over time (two-way ANOVA for dose, P < 0.0001), whereas triazole 1.1 (right) has no effect (P > 0.05). n = 4 to 11 mice per dose and treatment. (B) The total number of beam breaks observed in 1 hour significantly differed between U50,488H and triazole 1.1 treatment. Two-way ANOVA for interaction of dose and treatment: F2,28 = 12.54, P = 0.0083; Bonferroni post hoc analysis: ***P < 0.001, ****P < 0.0001 compared to triazole 1.1; U50,488H effects significantly differed from vehicle (interaction of dose and treatment: F1,44 = 66.86, P < 0.0001; Bonferroni post hoc analysis: ####P < 0.0001 compared to vehicle). n = 4 to 11 mice per dose and treatment. (C) KOR-KO mice did not differ in their responses to U50,488H (30 mg/kg, sc) and vehicle whether analyzed over time or as the summation of activity over the hour (two-way ANOVA for drug effect: P < 0.05, n = 4 to 7 mice per treatment). Data are means ± SEM.