Fig. 4. U50,488H but not triazole 1.1 decreases dopamine concentrations in the nucleus accumbens in mice.
(A and B) Cyclic voltammetric measures of dopamine release were made in slices of mouse brain in the nucleus accumbens. Triazole 1.1 did not induce decreases in dopamine release (nonconvergence of curves), whereas U50,488H did in a dose-dependent manner in both the core (A) [EC50 = 129 nM (95% CI, 51 to 230)] and the shell of the nucleus accumbens (B) [EC50 = 38 nM (95% CI, 9 to 157)]. Two-way ANOVA for dose and drug interaction: P = 0.0009; Bonferroni post hoc analysis: *P < 0.05, **P < 0.01, ***P < 0.001. The basal concentrations of dopamine (DA) release and rate of clearance are provided for each section in the bar charts. Data are means ± SEM of six to seven mice per treatment. (C) Microdialysis in nucleus accumbens of freely moving mice confirms a decrease in dopamine in response to U50,488H (3 mg/kg, ip), whereas triazole 1.1 (15 mg/kg, ip) did not decrease the baseline and was significantly different from U50,488H (P < 0.05, two-way ANOVA). n = 6 to 7 mice per treatment.