TABLE 6.
C1-controlled operators of P1
| Operatora (former name[s]) | Positionb | Strand | DNA sequencec | Genes controlled | Evidence for:
|
Reference(s) | |
|---|---|---|---|---|---|---|---|
| C1 binding | Regulatory function | ||||||
| Oref (Op1, Op2a) | 1962 | + | ATTGCTCTAATTGATTC | ref | √ | √ | 84, 337 |
| Omat (Op2, Op2b) | 2628 | + | CATGCACTAATAAATAT | mat | √ | √ | 84 |
| Ossb (Op21) | 19816 | + | ATTGCTCTAATTAATTT | ssb, isaA | √ | √ | 59 |
| Oc4 (Op51) | 46941 | + | AATGCTCTAATAAATTT | c4, icd, ant | √ | √ | 26, 84, 124, 316 |
| OkilA (Op53) | 48611 | + | TTTGCTCTAATAAATTT | kilA, repL | √ | 27, 84, 316 | |
| Orlf (Op55) | 50376 | + | ATTGCTCTAATTATAAC | rlfA, rlfB | √ | 112; reviewed in reference 346 | |
| Odmt (Op68) | 63946 | + | ATTGCTCTAATAAATAT | uhr, hrdC, dmt | √ | √ | 59 |
| Obanab (Op72, Op72ab) | 67468 | +/− | trnA, trnT, tciA, tciB, tciC, trnI, ban, dbn | √ | √ | 84, 129, 315, 317 | |
| a | 67468 | + | ATTGCTCTAATAAATTT | √ | 132, 316 | ||
| b | 67479 | − | ATTACACTAATAAATTT | √ | |||
| O26 (Op82) | 77329 | − | ATTGCACTAATAATTTT | 26, 25, 7, 24, 6, 5 | √ | Reviewed in 346 | |
| Oppp (Op83ab) | 78236 | − | CATGCACTAATTAATTT | pmgN, pmgO, pmgP, ppp, pmgQ | √ | Reviewed in reference 346 | |
| OpmgRa (Op86a) | 80953 | + | ATTGCTCTAATAATTCC | pmgR, pmgS | √ | 315, 346 | |
| OpmgRb (Op86, Op86b) | 81002 | − | ATTGCTCTAATAAAAAA | pmgR, pmgS | √ | 84, 316 | |
| OpmgT (Op88) | 83094 | + | ATTGCTCTAATAAATCT | pmgT, pmgU, pmgV | √ | √ | 59 |
| Olpa (Op94) | 88705 | + | ATTGCTCTAATGTATTG | lpa | √ | √ | 193 |
| OclabΛ (Op99ab, Op99a) | 92967 | −/+ | c1 | √ | √ | 27, 84, 128, 316, 317 | |
| a | 92978 | − | AATGCACTAATAAATCT | √ | 84, 316 | ||
| bd | 92967 | + | ACGAAAATAATAGATTT | √ | 317 | ||
| Oc1cΛ (Op99c) | 93150 | − | ATTGCTCTAACGCTTTA | c1 | √ | √ | 84, 128 |
| OcoiΛ (Op99d) | 93442 | − | ATTGCTCTAATAAATTA | c1, coi | √ | √ | 27, 128, 316 |
| OcreΛ (Op99e) | 93525 | + | ATTGCTCTAATAATTCT | cra, cre | √ | 27, 128, 316 | |
| Consensus | ATTGCTCTAATAAATTT | ||||||
The name of each operator refers to the name of a selected gene under its control. Bivalent operators names are underlined; constituent monovalent operators are listed below the name of each bivalent operator. Operators with a superscript Λ have been shown to engage in looping to nearby operators in vitro.
Position of the first nucleotide if the operator is assigned to the + strand or to the first nucleotide of its complement if the operator is assigned to the − strand. Coordinates refer to positions of operators in the P1 c1-100 mod749::IS5 genome without its nonintegral parts, IS5, and the associated 4-bp duplication.
The underlined nucleotides are conserved in monovalent operators and in at least one constituent operator of each bivalent operator.