Figure 5.

Representative pressure-area isotherms taken with different concentrations of WT GsMTx4 in the subphase. Qualitatively similar interactions of WT GsMTx4 with POPG-POPC monolayers at different concentrations are illustrated by compression isotherms. Visible monolayer “swelling” is observed at subphase peptide concentrations of 0.1 μM and above. The peptide tightly binds to the expanded monolayer. There is no obvious effect of gradual “squeezing-out” of the peptide from the monolayer at higher pressures. The monolayer instead has a tendency to collapse earlier (arrows) as the peptide concentration increases and pressure approaches 37–40 mN/m. In the control, the monolayer collapsed at 43–45 mN/m. The statistics are presented in Table 1. To see this figure in color, go online.