FIGURE 3:

The CC1 region of Par3 is necessary but not sufficient to drive apical membrane remodeling. (A) Confocal XZ-slices of MDCK cells expressing PH-Akt-GFP (PIP₃; green) transfected with AP-FRB and FK-∆CC1 (red; HA) and treated with vehicle (–Rapa) or 200 nM Rapalog (+Rapa) for 60 min. Actin was visualized with phalloidin staining (blue). (B) Quantitation of the appearance of apical PIP₃ and actin rearrangement in cells transfected with the indicated constructs in the presence or absence of Rapalog. (C) Confocal XZ-slices of cells expressing FK-CC1. (D) Quantitation of the appearance of apical PIP₃ and actin rearrangement in cells transfected with the indicated constructs in the presence or absence of Rapalog. Error bars, SEM. The p value was determined by one-way ANOVA followed by post hoc Tukey’s test. n = 3. Scale bar, 10 µm.