FIGURE 5:

PI3K and Tiam1 are sufficient to drive apical membrane remodeling. (A–C) Confocal XZ-slices of MDCK cells expressing PH-Akt-GFP (PIP₃; green), stained for actin (blue; phalloidin), and transfected with AP-FRB and (A) FK-iSH2 (red; HA), (B) FK-Tiam1 (red; HA), or (C) both constructs (red, FK-iSH2; cyan, FI-Tiam1) and treated with vehicle control (–rapa) or 200 nM Rapalog (+rapa) for 60 min. (D) Quantitation of immunofluorescence of apical PIP₃ or apical actin rearrangement for the indicated constructs and treatments. Error bars, SEM. The p value was determined by one-way ANOVA followed by post hoc Tukey’s test. n ≥ 3. Scale bar, 10 µm.