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. 2017 Jan 15;28(2):309–321. doi: 10.1091/mbc.E15-11-0759

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© 2017 Mattie et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Vacuole membrane docking and hemifusion visualized by transmission electron microscopy. (A) Transmission electron micrograph of a vacuole fusion reaction under control conditions (+ATP) at 30 min. Boxes are higher-magnification images illustrating a docking site between apposing organelles. Scale bars, 200 nm. Images of vacuoles incubated without ATP (B) or with ATP and recombinant Gdi1 protein, a Rab-GTPase inhibitor (14 µm; C) are shown as negative controls. Scale bars, 200 nm. (D) Top, transmission electron micrographs of docked vacuole membranes, a hemifusion diaphragm, and ruptured diaphragm. Bottom, higher-magnification images of membrane interfaces. Scale bars, 500 nm. (E) Averages of linear density plots within the areas shown in D (n = 10–88; error bars represent SD). Scale bars, 50 nm.