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. 2017 Jan 15;28(2):322–332. doi: 10.1091/mbc.E16-11-0764

TABLE 1:

Strains used in this study.

Strain Genotype Reference
BY4727 MATalpha his3∆200 leu2∆0 met15∆0 trp1∆63 ura3∆200 leu2∆0 met15∆0 trp1∆63 ura3∆0 Brachmann et al., 1998
BY4732 MATa his3∆200 met15∆0 trp1∆63 ura3∆0 Brachmann et al., 1998
BY4741 MATa his3∆1 leu2∆0 met15∆0 ura3∆0 Brachmann et al., 1998
CUY1953 BY4732 VPS41::TRP1-GAL1pr VPS41::TAP-URA3 VPS39::KanMX-GAL1pr VPS33::HIS3-Gal1pr Ostrowicz et al., 2010
CUY2489 BY4727 VPS11::HIS3-GAL1Pr VPS16::natNT2-GAL1Pr VPS18::kanMX-GAL1Pr-3HA Ostrowicz et al., 2010
CUY2675 CUY1953xCUY2489 Ostrowicz et al., 2010
CUY3396 BY4727 VPS11::HIS3-GAL VPS39::CloNat-GAL VPS39::TAP-URA3 VPS18::TRP1-GAL1 Bröcker et al., 2012
CUY3799 BY4732 VPS39::KanMX-Gal1Pr VPS33::HIS3-GAL1pr GAL1pr::pRS406-GAL1pr-VPS41(356-379∆)-URA3 VPS41::TAP-TRP This study
CUY3802 CUY2489xCUY3799 This study
CUY4605 CUY4640xCUY4641 This study
CUY4640 BY4727 VPS11::HIS3-GAL1pr VPS16::natNT2-GAL1pr VPS18::kanMX-GAL1pr-3HA VPS33::TRP1-GAL1pr This study
CUY4641 BY4741 VPS3::kanMX-Gal1pr VPS41::NatNT2-GAL1pr VPS41::TAP-URA3 This study
CUY5659 BY4732 VPS39::KanMX-Gal1Pr VPS33::HIS3-GAL1pr GAL1pr::pRS406-GAL1pr-Vps41 S-A-URA3 VPS41 S-A::TAP-TRP1 This study
CUY5666 CUY5659xCUY2489 This study
CUY8078 BY4732 VPS39::KanMX-Gal1Pr VPS33::HIS3-GAL1pr GAL1pr::pRS406-Gal1pr-VPS41 S-D-URA3 VPS41 S-D::TAP-TRP1 This study
CUY8243 CUY8078xCUY2489
CUY8292 BY4727 VPS11::HIS3-GAL1pr VPS16::natNT2-GAL1pr VPS18::kanMX-GAL1pr-3HA vps41∆::hphNT1 This study
CUY8356 BY4732 VPS39::KanMX-Gal1pr VPS33::HIS3-GAL1pr VPS41(1-499∆)::CloNAT-GAL1pr vps41∆aa1-499-TAP::URA This study
CUY8367 CUY8356xCUY8292 This study
CUY10111 BY4732 VPS41::TRP1-GAL1pr VPS33::HIS3-GAL1pr GALpr::pRS406-GALpr-VPS39(347-351∆)-TAP-URA3 This study
CUY10122 CUY10111xCUY2489 This study
CUY10153 BY4732 VPS41::TRP1-GAL1pr VPS33::HIS3-GAL1pr GAL::pRS406-GAL1pr-VPS39-TAP This study
CUY10154 CUY10153xCUY2489 This study
CUY10043 BY4727 vps39∆::hphNT1 NOP1::pRS406-NOP1pr-VPS39-GFP This study
CUY10045 BY4727 vps39∆::hphNT1 NOP1pr::pRS406-NOP1pr-VPS39(L347A)-GFP This study
CUY10046 BY4727 vps39∆::hphNT1 NOP1::pRS406-NOP1pr-VPS39(Q349A)-GFP This study
CUY10056 BY4727 vps39∆::hphNT1 NOP1::pRS406-NOP1pr-VPS39(347-351∆)-GFP This study
CUY10054 BY4727 vps11::HIS3-GAL URA3::pRS406-GAL1pr-Vps39-TAP This study
CUY10155 BY4727 vps11::HIS3-GAL GAL::pRS406-GAL1pr-VPS39(347-351∆)-TAP This study
CUY10385 CUY4605 VPS41(1-499∆)::hphNT1-GAL1pr This study
CSY9 vps11∆::HIS3MX6 pep4∆::KanMX6 VPS11-1ts-URA3 Stroupe et al., 2006
CSY10 vps11∆::HIS3MX6 pho8∆::KanMX6 VPS11-1ts-URA3 Stroupe et al., 2006