FIG. 5.

The genes of the hmg locus in H. influenzae strains. Panel A, representation of the locus in strain Rd. Arrows represent the relative location and the direction of transcription of each of the reading frames given in The Institute for Genomic Research database (http://www.tigr.org), with the appropriate designation (HI number) given above. The relative gene organization of the central portion of the hmg sequence for nontypeable H. influenzae strain 176 is given below for comparison, with the relevant gene designation. glnA and pepB are the flanking genes. For Rd, regions of DNA outside recognized reading frames that show some homology to O-antigen genes from other organisms are shown as dotted lines. The small square downstream of HI0870 is a tract of a repeated pentanucleotide (5′-TTATC). Panel B shows the LPS profiles for nontypeable H. influenzae strain 176 and the respective wbaP mutant. wt, wild type. Panel C shows the higher-molecular-weight glycoforms in the LPS of nontypeable H. influenzae strain 375 transformed with the hmg locus, compared to the wild type, after gel electrophoresis and staining with silver. −, represents before treatment with neuraminidase; +, after treatment with neuraminidase.